Analytical Techniques and Automation (6 cr)

Objectives (course unit)

The student is able to
-use absorption photometer and do its check measurements
-know the reaction principles of the kinetics methods in absorption photometry, calculation of the results
-know the basic principles of fluorometry, turbidimetry, nefolometry, luminometry and reflectance photometry, instrumentation and applications
-know the basics of electrophoresis and applications
-know the basics of electrochemical analytical methods, potentiometry, ISE electrodes, oxygen electrode
-know the basics of flow cytometry, chromatography, mass spectrometry and applications
- explain how antibodies are produced and how monoclonal and polyclonal antibodies differ from each other
- explain the principles of clinically most important immunoanalytical methods and describe the factors that affect the quality
- perform simple analyses using immunoanalytical methods, explain their clinical signifigance, preanalytical requirements, test principles and assess the reliability of the results

Content (course unit)

Check measurements of photometers
Kinetic measurements in absorption photometry
Fluorometry, turbidimetry, nefelometry, luminometry, reflectance photometry
Electrophoresis
Electrochemistry
Flow cytometry
Chromatography
Mass spectrometry
Immunoanalytical methods

Prerequisites (course unit)

Student must have passed 7B00BB84 before the course.

Exam schedules

Immunological methods 8.5.2015. Re-exams 26.8. and 16.9.

Assessment scale

0-5

Further information

The course includes training in the university of applied sciences' teaching laboratory

Assessment criteria - satisfactory (1-2) (Not in use, Look at the Assessment criteria above)

The student knows the main principles of clinically most important immunoanalytical methods. The student is able to explain on basic level how the of antibodies are produced and how the different kinds of antibodies differ from each other. Students are able to perform simple immunoanalytical assays with guidance, know their clinical importance, the most important pre-analytical requirements and main features of the interpretation of the results .

Assessment criteria - good (3-4) (Not in use, Look at the Assessment criteria above)

Students are able to explain the principles of clinically most important immunoanalytical methods and know the factors affecting their quality. The student is able to explain how the antibodies are produced and how the different antibodies differ from each other. Students are able to perform simple immunoanalytical assays according to written instructions with minimal guidance, and know their clinical importance, the most important pre-analytical requirements, the principle of the analysis method and interpretation of the results .

Assessment criteria - excellent (5) (Not in use, Look at the Assessment criteria above)

Students are able to explain the principles of clinically most important immunoanalytical methods and describe the factors affecting the quality. The student is able to explain in detail how the antibodies are produced and how the different antibodies differ from each other. The student can independently according to written instructions perform simple immunoanalytical assays, and can explain their clinical significance and pre-analytical requirements, the principle of the analytical method and the interpretation of the results .