Analytical Techniques and AutomationLaajuus (6 cr)

Objectives

The student is able to
-use absorption photometer and do its check measurements
-know the reaction principles of the kinetics methods in absorption photometry, calculation of the results
-know the basic principles of fluorometry, turbidimetry, nefolometry, luminometry and reflectance photometry, instrumentation and applications
-know the basics of electrophoresis and applications
-know the basics of electrochemical analytical methods, potentiometry, ISE electrodes, oxygen electrode
-know the basics of flow cytometry, chromatography, mass spectrometry and applications
- explain how antibodies are produced and how monoclonal and polyclonal antibodies differ from each other
- explain the principles of clinically most important immunoanalytical methods and describe the factors that affect the quality
- perform simple analyses using immunoanalytical methods, explain their clinical signifigance, preanalytical requirements, test principles and assess the reliability of the results

Content

Check measurements of photometers
Kinetic measurements in absorption photometry
Fluorometry, turbidimetry, nefelometry, luminometry, reflectance photometry
Electrophoresis
Electrochemistry
Flow cytometry
Chromatography
Mass spectrometry
Immunoanalytical methods

Prerequisites

Student must have passed 7B00BB84 before the course.

Further information

The course includes training in the university of applied sciences' teaching laboratory