Cell Culture Methods and Clean Room Work (3 cr)

Objectives (course unit)

The student is able to
- explain the essential terms and principles of cell culture and clean room techniques
- describe the ranges of use in clinical and research laboratories and methods that involve cell culture
- work aseptically and safely in cell culture and clean room laboratories
- maintain, analyze with microscopy and store cultured cells and count cells with hemocytometer
- define the requirements of GMP regulations in aseptic production
- gown to cleanroom, work aseptically and safely in cleanroom and take surface and air samples to monitor the cleanliness
- apply the knowledge and skills in further studies in diffent fields of biomedical sciences and in cleanroom work

Content (course unit)

Terms and theoretical background of cell culture
Ranges of use
Cell culture equipment
Basic methods of cell culture
Behavior of cells in culture, handling and maintaining cells
Primary cells/cell lines
Contamination/asepticity
Special techniques
Clean room work
GMP regulations
Practical cleanroom training, gowning and asepticity

Prerequisites (course unit)

The student has passed 13SBIO-1025 and 13SBIO-1026 as applicable before entering this course

Exam schedules

25.9.2017
Retake exams on general exam days 14.11. and 12.12.2017.

Evaluation methods and criteria

Participation to laboratory exercises (cell culture and cleanroom work) is compulsory.
Exam: graded 0-5

Assessment scale

0-5

Teaching methods

lectures
laborations
exam

Learning materials

Lecture material.
Forss, Heinonen: Soluviljelijän käsikirja. Oppimateriaalia soluviljelymenetelmäkurssille.
Additional material for example
Butler, M . 2007. Cell Culture and upstream processing. New York, N.Y. Taylor & Francis Group .
Prabhakar, U., & Kelley , M. 2008. Validation of cell -based assays in the GLP setting: a practical guide. Chichester: Wiley.
Davis, J. M. 2002. Basic Cell Culture: a practical approach. Oxford University Press.
Freshney R. Ian. 2005. Culture of animal cells: a manual of basic technique. New York: Wiley -Liss.
Helgason, Miller. 2005. Basic Cell Culture Protocols. Humana Press.
Sullivan, S. Cowan , C . & Eggan K . 2007. Human embryonic stem cells : the practical handbook . Chichester : John Wiley & Sons .

Student workload

Lectures 26 h + 2 h exam .
Laboratory 7 h cell culture and 3 h cleanroom work.
Independent work approximately 43 h.

Content scheduling

Lectures: Tuuli Välineva and Leila Kakko
Cell Culture Labs: Janette Hinkka
Cleanroom Working Exercises: Tuuli Välineva and Leila Kakko

Further information

The course includes training in the university of applied sciences’ teaching laboratory

Assessment criteria - fail (0) (Not in use, Look at the Assessment criteria above)

Compulsory tasks are missing.

Assessment criteria - satisfactory (1-2) (Not in use, Look at the Assessment criteria above)

The student knows the main principles of cell culture and clean room work. He can explain the main terms and concepts. The student knows the areas of use of cell culture and clean room work most relevant for biomedical technologists. The student can work aseptically and safely in cell culture laboratory or clean room. The student can maintain and handle cultured cells with guidance, and follow clean room hygiene with surface and air sampling.

Assessment criteria - good (3-4) (Not in use, Look at the Assessment criteria above)

The student can extensively interpret the terms and concepts of cell culture and clean room work. He can explain the behavior of animal cells in culture and name the factors needed for their growth. The student knows the areas of use of cell culture and clean room work. The student can work aseptically and safely in cell culture laboratory or clean room and apply the theoretical knowledge to laboratory work. The student can maintain and handle cultured cells according to written instructions, plan cell culture experiments and follow clean room hygiene with surface and air sampling.

Assessment criteria - excellent (5) (Not in use, Look at the Assessment criteria above)

The student can extensively interpret the terms, concepts and principles of cell culture and clean room work. He can explain the behavior of animal cells in culture and name the factors needed for their growth and explain their mechanisms. The student knows the areas of use of cell culture and clean room work. The student can work aseptically and safely in cell culture laboratory or clean room and apply the theoretical knowledge to laboratory work. The student can independently search for information and according to that maintain and handle different kinds of cultured cells and plan cell culture experiments. The student is able to follow clean room hygiene with surface and air sampling and interpret the results from different kinds of hygiene monitoring samples.