Study Guide

Objectives

The core objective of the course is that the student learns the basics of cell culture methods and cleanroom work both in theory and practice.

After completion of the course the student:
• can explain the essential terms, application areas, principles and requirements of cell culture and clean room work
• can explain the basic techniques of cell culture
• works aseptically and safely in cell culture laboratory
• is able to maintain cultured cells using basic techniques

Content

• Cell culture principles, application areas and basic concepts
• Cell culture conditions
• Cell culture laboratory equipment
• Safety in cell culture work
• Basic cell culture methods, cell handling and maintenance
• Contaminations and aseptic work
• Cleanroom classification, basics of cleanroom technology and cleanroom work
• Cell culture practicals in the teaching laboratory
• Clean room simulation exercises

Assessment criteria, satisfactory (1-2)

The student can explain some of the most important principles and key concepts of cell culture and cleanroom work. The student can name some application areas of cell culture and cleanroom work, which are central to the work of a biomedical laboratory scientist. The student knows the main features of how animal cells are cultured in the laboratory and what kind of growth requirements they have. The student knows how to work aseptically and safely in cell culture and cleanrooms. The student is able to participate in maintaining and handling conventional cell cultures under supervision.

Assessment criteria, good (3-4)

The student can explain the most important principles and concepts of cell culture and cleanroom work. The student knows the key application areas of cell culture and clean room work in terms of the work of a biomedical laboratory scientist. The student can explain how animal cells are cultured in the laboratory and what kind of growth requirements they have. The student knows how to work aseptically and safely in cell culture and cleanrooms and knows how to use his theoretical knowledge in his work. The student is able to maintain and handle conventional cell cultures according to written instructions under guidance.

Assessment criteria, excellent (5)

The student can comprehensively explain the concepts and principles of cell culture and cleanroom work. The student knows the key application areas of cell culture and clean room work in terms of the work of a biomedical laboratory scientist. The student can explain how animal cells are cultivated in the laboratory and what kind of growth requirements they have. The student can explain how animal cells are cultured. The student can describe the behavior of cells in a culture, name the factors necessary for cell growth and explain their effects. The student knows how to work aseptically and safely in cell culture and cleanrooms and knows how to apply his theoretical knowledge in his work. The student can independently search for information on the topics covered. The student is able to maintain and handle conventional cell cultures mainly using written instructions.

Further information

The course includes practical training in the teaching laboratory.