Cell Culture Methods and Clean Room WorkLaajuus (3 cr)

Objectives

The student is able to
- explain the essential terms and principles of cell culture and clean room techniques
- describe the ranges of use in clinical and research laboratories and methods that involve cell culture
- work aseptically and safely in cell culture and clean room laboratories
- maintain, analyze with microscopy and store cultured cells and count cells with hemocytometer
- define the requirements of GMP regulations in aseptic production
- gown to cleanroom, work aseptically and safely in cleanroom and take surface and air samples to monitor the cleanliness
- apply the knowledge and skills in further studies in diffent fields of biomedical sciences and in cleanroom work

Content

Terms and theoretical background of cell culture
Ranges of use
Cell culture equipment
Basic methods of cell culture
Behavior of cells in culture, handling and maintaining cells
Primary cells/cell lines
Contamination/asepticity
Special techniques
Clean room work
GMP regulations
Practical cleanroom training, gowning and asepticity

Prerequisites

The student has passed 13SBIO-1025 and 13SBIO-1026 as applicable before entering this course

Further information

The course includes training in the university of applied sciences’ teaching laboratory