Histological Stains, Immunohistochemistry and Molecular Pathology (3 cr)

Objectives (course unit)

The students
- know the theoretical background of the most common histological stains, immunohistochemistry, and molecular pathology
- identify the most common histological stains by microscope and digital imaging
- are familiar with quality assurance and occupational safety in staining
- know post-mortem practices

Content (course unit)

Most common histological stains
Methods of immunohistochemistry and molecular pathology
Microscopic recognition of histological stains
Sources of error in stainings, quality assurance
Occupational safety
Clinical and forensic post-mortem examinations

Prerequisites (course unit)

The student must have passed 7B00EG29 Preparation of Tissue Sections and Recognition of Tissues, and 7B00EG30 Introduction to Clinical Cytology before the course.

Assessment criteria, satisfactory (1-2) (course unit)

The student knows the principles and clinical use of the main histological and immunohistological stains and molecular pathology. He/she knows the principles of quality assurance of these methods and occupational safety. The student can identify some of the most common histological stains by microscopy.

Assessment criteria, good (3-4) (course unit)

The student knows the principles and clinical use of the main histological and immunohistological stains and molecular pathology. He/she knows the principles of quality assurance of these methods and occupational safety. The student can identify the most common histological stains by microscopy.

Assessment criteria, excellent (5) (course unit)

The student knows the principles and clinical use of the main histological and immunohistological stains and molecular pathology. He/she can evaluate quality assurance of these methods and occupational safety. The student can identify the most common histological stains by microscopy.

Assessment criteria, pass/fail (course unit)

-

Location and time

Info ja värjäysten periaatteet 2 t (Eeva ½ h ja Essi 1 h) 4.1.24
Immunohistokemia ja molekyylipatologia 6 h (Menetelmät Essi 12.1., Eeva 15.1, Eeva 18.1)
Erikoisvärjäysten esittely ja niistä keskustelu 4 h (Essi 22.-23.1)
Biopankkitoiminta ja Kuolemansyyn selvittäminen 2 h (29.1)
Erikoisvärjäyspaketit ja värjäysten laadunhallinta 2 t (Eeva värjäyspaketit ½ h ja Essi värjäysten laadunhallinta 1½ h) 30.1.
Värjäysten mikroskopointi 4 t/pienryhmä opetuslaboratoriossa 7.2. (Eeva ja Essi)
Immunovärjäysten mikroskopointi 2 t/pienryhmä opetuslaboratoriossa 14.2. (Eeva ja Essi)
Tentti (1½ h) ja tentin palaute ½ h) 19.2.24

Exam schedules

Tentti 19.2.24
Korotus ja ensimmäinen uusintatentti Examissa 18.-23.3.24
Toinen uusintatentti Examissa 15.-20.4.24

Assessment methods and criteria

Tentti

Assessment scale

0-5

Teaching methods

Lectures, lavoratory work, seminars, independent work

Learning materials

Moodlessa oleva materiaali
Virtuaalimikrosoopin materiaali
MUUTA MATERIAALIA
DAKO:n oppimateriaalit
Naukkarinen, A. Histologiset menetelmät 2003 tai uudempi painos. Kuopion yliopisto. Koulutus- ja kehittämiskeskus. Osat: Histologisten värjäysten teoriaa. Hematoksyliini – ja Wegert van Gieson –värjäykset. Hiilihydraattien osoittaminen kudosleikkeestä. Histologisia erikoisvärjäyksiä
Bancroft J & Gamble M (toim). 2002. Theory and Practice of Histological Techniques. Churchill Livingstone.
Orchard G & Nation B. 2012. Histopathology. Oxford University Press. Sivut 127-169.
Dey P. 2018. Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Springer. Sivut 81-118. ekirja
http://earlydetectionofbreastcanser.weebly.com/

Student workload

Kontatkiopetus 22 t
Tentti ja tentin palaute 3 t
Itsenäinen opiskelu 56 t

Content scheduling

Läsnäolo pakollinen kaikilla tunneilla.

Completion alternatives

-

Practical training and working life cooperation

-

International connections

-

Further information

The course includes training in the university of applied sciences' teaching laboratory.