Cell Culture MethodLaajuus (2 cr)

Content

Type of course
Compulsory

Level of course
Professional studies

Objective of course
The student:
-knows the basic concepts of cell culture techniques
-knows the ranges of use and research methods of cell culture
-knows the practical working methods in cell culture laboratories

Course contents
-terminology and theory of cell culture
-use of cell culture
-equipment of cell culture laboratories
-basic methods in cell culture
-behaviour of cells in cell cultures, handling and maintenance
-primary cells/cell lines
-contamination / aseptics
-special techniques

Teaching methods
Lectures, independent study, supervised clinical training in the teaching laboratory / study visit

Assessment methods
Participation in teaching, passed written assignments, graded examination

Prerequisites
-

Recommended reading
-Butler,M. 2007. Cell Culture and upstream processing. New York, N.Y. : Taylor & Francis Group.
Prabhakar, U. & Kelley, M.2008. Validation of cell-based assays in the GLP setting: a practical guide. Chichester : Wiley.
Davis, J.M. 2002. Basic Cell Culture: a practical approach. Oxford University Press.
Freshney R. Ian. 2005. Culture of animal cells: a manual of basic technique. New York: Wiley-Liss.
Helgason, Miller. 2005. Basic Cell Culture protocols. Humana Press.
Sullivan, S.& Cowan,C.& Eggan,K. 2007.Human embryonic stem cells : the practical handbook. Chichester : John Wiley & Sons.

Name of lecturers
FT Tuuli Välineva


Year of study
Second academic year

Semester
Autumn periods 1-2

Language of instruction
Finnish